Void Volume, Dead Volume and Dwell Volume In HPLC: Learn Easily In 11 Minutes - PharmaGuru | Global Pharma Training, Certifications & Expert Blogs

Q: What is dwell volume in HPLC?

Dwell volume (also known as gradient delay volume and denoted as Vᴅ ) is the volume of mobile phase between the solvent mixing point and the column inlet in a gradient system

Q: What is the dead volume in HPLC?

Dead volume refers to the volume of mobile phase between the injection point and the detector, excluding the void volume of the column. It includes all system components

Question 1

What is dwell volume in HPLC?

Accepted Answer

Dwell volume (also known as gradient delay volume and denoted as Vᴅ) is the volume of mobile phase between the solvent mixing point and the column inlet in a gradient system

Question 2

What is the dead volume in HPLC?

Accepted Answer

Dead volume refers to the volume of mobile phase between the injection point and the detector, excluding the void volume of the column. It includes all system components

Question 3

What is a void peak in HPLC?

Accepted Answer

The peak due to void volume is called the void volume peak or unretained peak. it is denoted by to

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By Dr Pramod Kumar Pandey - June 6, 2025

Protected: Void Volume, Dead Volume and Dwell Volume In HPLC: Learn Easily In 11 Minutes

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