HPLC Method Development Training | Course

Description

HPLC Method Development Training | Course

PharmaGuru HPLC Method Development Training equips analysts with practical skills to develop, optimise, and validate robust HPLC methods, covering column chemistry, mobile phase selection (pH, buffers), gradient optimisation, and regulatory guidelines with hands-on experience

Module 1: Introduction to HPLC

• Welcome to PharmaGuru
• Why is HPLC Required?
• Historical Background and Principles of HPLC
• Key Components of HPLC and Their Functions
• Peak Formation in HPLC
• Applications of HPLC
• Advantages and Limitations of HPLC
• Interactive Question & Answer Session
• Overview of PharmaGuru Online Courses

Module 2: Fundamentals of Chromatographic Separation

• Separation Mechanisms in HPLC
• Components of an HPLC Chromatogram
• Guard Filters, Guard Columns, and Ghostbuster Columns
• Important Chromatographic Parameters:
  • Theoretical Plates (N)
  • Resolution (Rs)
  • Tailing Factor (T)
  • Capacity Factor (K)
  • Selectivity (α)
• Role of Partition, Adsorption, & Ion-Exchange Mechanisms in Separation Efficiency
• HPLC Detectors and Their Applications
• Interactive Question & Answer Session

Module 3: HPLC Modes and Column Selection

• HPLC Modes: Reverse Phase Chromatography (RPC) and Normal Phase Chromatography (NPC)
• HPLC Column Chemistry and Selection Strategy
• Equivalent HPLC Columns and Their Applications
• Modes of Elution:
  • Isocratic Elution
  • Gradient Elution
• HPLC Column Equilibration Best Practices
• How to Set Up an HPLC System Efficiently
• Peak Integration in HPLC

Module 4: System Suitability and HPLC Calculations

• System Suitability Testing (SST):
  • Components
  • Acceptance Criteria
• Void Volume, Dead Volume, and Dwell Volume in HPLC
• Important HPLC Equations and Their Applications
• Various Calculation Approaches in HPLC Analysis
• Role of Relative Response Factors (RRF) in Impurity Calculations

Module 5: Mobile Phase Chemistry and Optimisation

• Mobile Phase Chemistry and Selection Strategy
• Mobile Phase Preparation: Best Practices and Common Mistakes
• Mobile Phase Modifiers and pH Selection
• Role of pKa in Reverse Phase HPLC Method Development
• HPLC Water Quality Requirements
• Common HPLC Solvents and Their Applications
• Understanding Ghost Peaks and the Role of Ghostbuster Columns

Module 6: HPLC Method Development Strategies

Assay Methods

• Step-by-Step Assay Method Development

Related Substances and Impurity Profiling

• Method Development for Related Substances
• Impurity Profiling Approaches

Chromatographic Modes

• Normal Phase Chromatography (NPC)
• Reverse Phase Chromatography (RPC)
• Ion-Pair Reverse Phase Chromatography (IPRPC)

• Sessions: 6 sessions (9 hours / 540 minutes each)

• Mode of Training: Online (via Google Meet)

• Certificate: Yes, a certificate of completion will be provided

• Money-Back Guarantee: 30-day money-back guarantee

• Outcome:

  • Strong foundational knowledge
  • Clear career direction
  • Improved decision-making for advanced learning

  Language: English

  Instructor: Dr Pramod Kumar Pandey (PhD in Chemistry) | 31+ years of experience in global and Indian pharmaceutical industries