Description
HPLC Column Chemistry | Foundation Course | Course Description
The HPLC Column Chemistry | Foundation Course by PharmaGuru is a beginner-level training program designed to help learners understand the fundamental role of column chemistry in high-performance liquid chromatography (HPLC). The course explains how different stationary phases influence separation, selectivity, and resolution in pharmaceutical analysis.
Learners will explore key column types, bonding chemistries, particle technologies, and selection criteria used in method development and optimization. This course is ideal for students and early-career professionals aiming to build a strong foundation in analytical method development and quality control.
Key Learnings
- Fundamentals of HPLC column chemistry and stationary phases
- Understanding silica-based and polymer-based column materials
- Overview of reverse phase, normal phase, and specialty columns
- Role of bonded phases and functional groups in separation
- Column particle size, pore size, and their impact on efficiency
- Column selection criteria for method development
- Factors affecting column performance and longevity
- Introduction to column troubleshooting and maintenance basics





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