Description
Fundamentals of HPLC: Free Training Course
Course Description
Build a solid foundation in High-Performance Liquid Chromatography (HPLC), one of the most widely used analytical techniques in pharmaceutical, chemical, and life science industries. This course introduces the core principles of liquid chromatography, explaining how compounds are separated using a liquid mobile phase and a solid stationary phase.
You’ll explore different HPLC modes—such as reverse-phase, normal-phase, size-exclusion, and affinity chromatography—and learn how to choose the right combination of column chemistry and mobile phase for your specific analytical needs. The course also covers practical method development, including how to optimise key parameters like flow rate, temperature, and detection settings to achieve reliable and reproducible results.
Finally, discover how HPLC is applied across the entire product lifecycle—from early-stage drug discovery and ADME profiling to formulation development and quality control of finished products.
Key Learning Outcomes
By the end of this course, you will be able to:
- Understand the fundamental principles of HPLC and how separation is achieved
- Differentiate between major HPLC modes (reverse-phase, normal-phase, size-exclusion, affinity) and identify their appropriate applications
- Select suitable columns, mobile phases, and detectors based on analyte properties
- Optimize critical method parameters to improve resolution, efficiency, and reproducibility
- Troubleshoot common HPLC issues and refine analytical methods
- Apply HPLC techniques across various stages of product development, including drug discovery, formulation, and quality control
- Interpret chromatographic data to make informed analytical decisions
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