Chiral Separation – Advanced Training | By HPLC & GC

Description

Chiral Separation – Advanced Training | By HPLC & GC

Chiral Method Development Using Polysaccharide, Cyclodextrin, Crown Ether, Pirkle-Type, Protein-Based & Macrocyclic Antibiotic Chiral Stationary Phases (CSPs)

The PharmaGuru Chiral Separation Masterclass is a comprehensive, industry-oriented training program designed for analytical scientists, chromatographers, pharmaceutical researchers, and regulatory professionals seeking advanced expertise in chiral method development using both HPLC and GC.

This intensive program provides a systematic framework for developing robust, efficient, and regulatory-compliant chiral methods. Participants will learn how to select the right separation technique, stationary phase, mobile phase, and optimisation strategy for simple as well as highly complex chiral molecules.

The training combines fundamental principles, practical workflows, industrial case studies, troubleshooting strategies, and regulatory expectations to help participants confidently address real-world chiral separation challenges.

What Makes This Program Unique?

• Covers both Chiral HPLC and Chiral GC
• Structured decision-making approach for rapid method development
• Comprehensive coverage of all major chiral stationary phase technologies
• Strategies for molecules containing up to six chiral centres
• Regulatory expectations for chiral impurity control
• Real-world pharmaceutical case studies
• Cost-reduction and efficiency improvement strategies
• Practical troubleshooting based on industrial experience
• Includes the complete content of both:
  • Chiral Separation Foundation Course
  • Chiral Separation Advanced Course

Training Modules

Module 1: Selecting the Right Chiral Separation Technique

Learn how to choose between Chiral HPLC and Chiral GC based on:

• Molecular structure and physicochemical properties
• Volatility and thermal stability
• Molecular weight and polarity
• Functional groups
• Regulatory requirements
• Practical decision trees and industrial examples

Module 2: Principles of Chiral Method Development

Develop a strong foundation in chiral recognition and separation science:

• Fundamentals of chirality
• Enantiomers, diastereomers, and stereoisomers
• Chiral recognition mechanisms
• Three-point interaction model
• Factors affecting enantioselectivity
• Key chromatographic parameters:
  • Selectivity (α)
  • Resolution (Rs)
  • Retention Factor (k’)
  • Efficiency (N)

Module 3: Mobile Phase Selection for Chiral HPLC

Master the selection and optimisation of mobile phases:

• Normal-phase mode
• Reversed-phase mode
• Polar-organic mode
• Heptane/alcohol systems
• Aqueous buffers and organic modifiers
• Solvent effects on selectivity, retention, and robustness

Module 4: Modifier Selection for Chiral HPLC

Learn how additives influence chiral separations:

• Alcohol modifiers
• Acidic additives
• Basic additives
• Polar modifiers
• Peak shape optimisation
• Enantioselectivity enhancement
• Modifier-related troubleshooting

Module 5: Selecting the Right Chiral Stationary Phase (CSP)

Comprehensive coverage of major CSP technologies:

HPLC Chiral Stationary Phases

• Polysaccharide-Based CSPs
• Cyclodextrin-Based CSPs
• Crown Ether CSPs
• Pirkle-Type CSPs
• Protein-Based CSPs
• Macrocyclic Antibiotic CSPs

Learn:

• Chiral recognition mechanisms
• Structure-based CSP selection
• Interaction mapping
• Rational screening strategies
• Column screening workflow design

Module 6: Selecting CSPs for Chiral GC

Develop effective GC screening strategies using:

• Cyclodextrin-based GC columns
• Derivatised cyclodextrin phases
• Specialised GC chiral stationary phases

Topics include:

• Volatility considerations
• Functional group compatibility
• Temperature limitations
• Column selection strategies
• GC method optimisation

Module 7: Structured Separation Strategies for Complex Chiral Molecules

Molecules with 1–6 Chiral Centres

Learn systematic approaches for:

• Single-centre chiral compounds
• Multi-centre stereochemical systems
• Complex pharmaceutical molecules
• Isomer identification and characterisation

Additional topics:

• Enantiomeric vs diastereomeric separations
• Racemisation mechanisms
• Factors affecting racemisation
• Impact of racemisation on method development
• Handling multiple peaks and co-elution
• Advanced column pairing strategies
• Multi-technique separation approaches
• Establishing specifications and limits for chiral impurities and isomers

Module 8: Reducing Analysis Cost in Chiral HPLC & GC

Practical strategies to improve efficiency and reduce costs:

• Reducing solvent consumption
• Minimising run time
• Extending column lifetime
• Cost-effective screening approaches
• Selecting economical CSPs and mobile phases
• Improving laboratory productivity

Module 9: Optimisation & Finalisation of Routine Chiral Methods

Transform screening methods into validated routine methods:

Optimisation Workflow

Screen → Refine → Optimise → Validate

Topics include:

• Robustness studies
• Intermediate precision
• System suitability
• Method lifecycle management
• Routine method implementation

Module 10: Regulatory Expectations & Chiral Method Development Reports

Learn how to prepare professional development reports and address regulatory expectations.

Documentation Requirements

• Column screening studies
• Mobile phase selection rationale
• Optimisation experiments
• System suitability justification
• Final method summary

Regulatory Topics

• Chiral impurity control strategies
• ICH Q6 perspectives on chiral control
• Pharmacopoeial approaches to chiral control
• Role of Specifications and SOR in chiral control
• Common Deficiency Letters (DLs) related to chiral methods and controls

Module 11: Case Studies, FAQs & Industrial Problem Solving

Apply your learning through:

15+ Detailed Industrial Case Studies

Covering:

• Single and multi-centre chiral molecules
• HPLC vs GC decision-making
• Difficult separations
• Poor selectivity challenges
• Co-elution troubleshooting
• Long runtime optimisation
• Chiral impurity control
• Cost-reduction projects

Interactive Problem-Solving Sessions

• Frequently asked questions
• Live troubleshooting discussions
• Expert recommendations
• Industrial best practices

Training Details

Duration

12 Live Sessions

Total Training Time

18 Hours (1080 Minutes)

Mode

Online Live Training via Google Meet

Target Audience

• Analytical Development Scientists
• Quality Control Professionals
• Quality Assurance Professionals
• Regulatory Affairs Professionals
• Research & Development Scientists
• Pharmaceutical Researchers
• Chromatographers
• Academicians and Research Scholars

Certificate

Certificate of Completion Provided

Money-Back Guarantee

30-Day Money-Back Guarantee

Language

English

Instructor

Dr. Pramod Kumar Pandey, PhD (Chemistry)

31+ years of experience in Indian and Global Pharmaceutical Industries with expertise in:

• Analytical Method Development & Analytical Method Validation
• Chiral Separations
• Regulatory Compliance
• Pharmaceutical Research & Development
• Quality Systems
• Chromatography Applications

Bonus

Participants will gain access to real industrial challenges, practical troubleshooting approaches, and expert solutions developed through more than three decades of pharmaceutical experience.

Special Note

This Masterclass incorporates the complete content and advanced learnings from both the Foundation and Advanced Chiral Separation Training Programs, making it one of the most comprehensive chiral separation courses available for pharmaceutical professionals.