LC-MS – Foundation Course

Description

Training Description: LC-MS – Foundation Course | Liquid Chromatography–Mass Spectrometry

This training program provides a comprehensive understanding of Liquid Chromatography–Mass Spectrometry (LC-MS), one of the most powerful analytical techniques used in pharmaceutical development, research, and quality control. Participants will learn the fundamental principles of chromatographic separation, mass spectrometric detection, ionization techniques, mass analyzers, data interpretation, and structure elucidation. The course also covers practical applications of LC-MS in impurity profiling, metabolite identification, degradation studies, bioanalysis, and quantitative analysis. Real-world case studies and industry-relevant examples are included to strengthen analytical problem-solving and interpretation skills.

Key Learning Outcomes

1. Session 1, Day 1

• LCMS Working Principle, procedure, Spectrum Representation, LCMS System Configuration & Mass Spectroscopic Terms, Mobile phase selection, Sample Preparation, Different Ionization Methods, ESI overview, Formation of Charged Droplets, positive ion mode (ESI+) and negative ion mode (ESI−), Pharmaceutical case studies,

2. Session 2, Day 2

• Mass Analyzer, Triple Quad Configuration and function, Collision Cell, Mass Resolution, Mass Resolution Accuracy, Procedure to Identify the Molecular Ion, SIM & MRM, Mass interpretation of molecules containing Cl, Br or S, Stevenson’s Rule & Fragmentation Mechanism, a-Cleavages & b-Cleavages, Structure Elucidation Procedure with case studies, Nitrogen Rule, Unknown Impurity Identification in reaction mixture or in pharmaceuticals with Case studies, Quantification of GIs, Applications and Limitatins of LCMS, LCMS interpretation of different pharmaceuticals

Target Audience

• Analytical Scientists
• Quality Control Analysts
• Pharmaceutical R&D Professionals
• Method Development Scientists
• Regulatory Affairs Professionals
• Research Scholars and Students in Pharmaceutical Sciences, Chemistry, and Biotechnology

Note: This training program is offered in flexible formats, including classroom (in-person), online courses (Foundation, Most Popular, and Advanced levels), as well as customised corporate training solutions for organisations.

Sessions: 2 sessions (3 hours / 180 minutes)

Target Audience: Analytical / QC / QA / RA / R&D

Mode of Training: Online (via Google Meet)

Certificate: Yes, a certificate of completion will be provided

Money-Back Guarantee: 30-day money-back guarantee

Language: English

Instructor: Dr Pramod Kumar Pandey (PhD in Chemistry) | 31+ years of experience in global and Indian pharmaceutical industries

Expected Competencies After Training

Participants will gain the ability to operate LC-MS concepts confidently, select appropriate analytical conditions, interpret mass spectra, identify impurities and degradation products, and apply LC-MS techniques effectively in pharmaceutical development and quality control environments.