Description

Ultimate Chiral Separation Training by HPLC 

Advanced Chiral Method Development Using Polysaccharide, Cyclodextrin & Crown Ether Stationary Phases

Master the science and strategy of chiral separations through this comprehensive industry-focused training program designed for analytical scientists, chromatographers, pharmaceutical researchers, and quality professionals. This advanced course provides a systematic approach to developing robust, cost-effective, and regulatory-compliant chiral methods using both HPLC and GC techniques.

Led by Dr. Pramod Kumar Pandey (PhD, Chemistry) with over 31 years of experience in global and Indian pharmaceutical industries, this program combines fundamental principles, practical method development workflows, real-world case studies, and expert troubleshooting strategies used in pharmaceutical R&D and quality control laboratories.

Whether you are new to chiral separations or looking to enhance your method development expertise, this training will equip you with the knowledge and confidence to tackle simple to highly complex chiral molecules.

What Makes This Training Unique?

• Complete coverage from chiral separation fundamentals to advanced method optimisation

• Structured decision-making approach for rapid method development

• Practical strategies for selecting columns, mobile phases, and modifiers

• Coverage of both Chiral HPLC and Chiral GC methodologies

• Real pharmaceutical case studies and troubleshooting examples

• Cost-reduction strategies for routine analytical operations

• Guidance on preparing professional chiral method development reports

• Includes all content from the Chiral Method Development Foundation Course

Training Modules

Module 1: Selecting the Right Chiral Separation Technique

Learn how to choose between Chiral HPLC and Chiral GC based on:

• Molecular structure and physicochemical properties

• Volatility and thermal stability

• Polarity and molecular weight

• Regulatory and analytical requirements

• Practical decision trees and real-world selection examples

Module 2: Principles of Chiral Method Development

Build a strong foundation in chiral recognition and separation science:

• Fundamentals of chirality and enantiomeric separation

• Mechanisms of chiral recognition

• Three-point interaction model

• Critical chromatographic parameters:

  • Selectivity (α)

  • Resolution (Rs)

  • Retention Factor (k’)

  • Column Efficiency (N)

• Factors influencing enantioselectivity

Module 3: Mobile Phase Selection for Chiral HPLC

Develop effective screening strategies for mobile phase optimisation:

• Normal-phase, reversed-phase, and polar-organic modes

• Selection of heptane/alcohol compositions

• Use of aqueous buffers and polar solvents

• Influence of mobile phase composition on selectivity, retention, and resolution

• Improving method robustness and reproducibility

Module 4: Modifier Selection for Chiral HPLC

Understand how additives influence chiral separations:

• Selection of alcohol modifiers

• Acidic and basic additives

• Polar modifiers and their applications

• Effects on peak shape, retention, and enantioselectivity

• Troubleshooting modifier-related chromatographic issues

Module 5: Selecting the Right Chiral Stationary Phase (CSP)

Polysaccharide, Cyclodextrin & Crown Ether Stationary Phases

Gain a systematic approach to CSP selection:

• Polysaccharide-based CSPs

• Cyclodextrin-based CSPs

• Crown Ether CSPs

• Pirkle-type CSPs

• Protein-based CSPs

• Macrocyclic antibiotic CSPs

• Structure-based column selection strategies

• Designing efficient column screening workflows

Module 6: Selecting CSPs for Chiral GC

Master stationary phase selection for gas chromatographic chiral separations:

• Cyclodextrin-based GC chiral columns

• Derivatised cyclodextrin phases

• Specialised GC chiral stationary phases

• Selection based on analyte volatility and functionality

• Temperature limitations and stability considerations

• Efficient GC screening strategies

Module 7: Structured Separation Strategies for Different Classes of Chiral Molecules

A. Molecules with One Chiral Centre

• Predicting chiral interactions

• Selecting suitable CSPs and mobile phases

• Rapid first-pass screening strategy

B. Molecules with Two Chiral Centres

• Understanding enantiomeric and diastereomeric separations

• Managing multiple stereoisomers

• Resolving co-elution challenges

C. Molecules with Multiple Chiral Centres (1, 2 & 3 Centres)

• Advanced strategies for complex pharmaceutical compounds

• Multi-column screening approaches

• Combining complementary separation techniques

• Optimising challenging separations

Module 8: Cost Reduction Strategies in Chiral HPLC

Learn practical approaches to reduce analytical costs without compromising performance:

• Minimising solvent consumption

• Reducing analysis time

• Extending column life

• Cost-effective column selection

• Efficient screening strategies

• Development of economical routine methods

Module 9: Optimisation and Finalisation of Routine Chiral Methods

Transform screening methods into robust routine procedures:

• Structured workflow:

  • Screen

  • Refine

  • Optimise

  • Validate

• Robustness studies

• Intermediate precision evaluation

• System suitability criteria

• Method lifecycle considerations

Module 10: Preparing a Chiral Method Development Report

Learn industry-standard documentation practices:

• Column screening summaries

• Mobile phase selection rationale

• Optimisation study documentation

• System suitability justification

• Final method reporting

• Regulatory expectations for pharmaceutical submissions

Module 11: Case Studies, FAQs and Real-World Problem Solving

Apply your learning through practical examples and industry challenges:

Detailed Case Studies Covering:

• Single chiral centre molecules

• Two chiral centre molecules

• Multiple chiral centre pharmaceutical compounds

• Poor selectivity issues

• Co-elution troubleshooting

• Long run time optimisation

• Cost-reduction success stories

Interactive Troubleshooting Sessions:

• Frequently asked questions

• Expert recommendations

• Real laboratory challenges and solutions

Training Details

Duration: 6 Live Online Sessions
Total Training Time: 9 Hours (540 Minutes)

Mode: Online Live Training via Google Meet

Certificate: Certificate of Completion Provided

Money-Back Guarantee: 30-Day Money-Back Guarantee

Language: English

Instructor: Dr. Pramod Kumar Pandey, PhD (Chemistry)
31+ Years of Experience in Global and Indian Pharmaceutical Industries

Learning Outcomes

Upon completion of this training, participants will be able to:

• Understand the fundamental principles of chiral recognition and separation

• Select appropriate chiral separation techniques for different molecules

• Choose suitable CSPs, mobile phases, and modifiers systematically

• Develop robust and efficient chiral HPLC and GC methods

• Troubleshoot complex separation challenges with confidence

• Reduce analytical costs while maintaining method quality

• Prepare professional chiral method development reports

• Implement validated routine chiral methods in pharmaceutical laboratories

Bonus

Exclusive discussion of real industrial chiral separation challenges, practical solutions, and expert insights gained from more than three decades of pharmaceutical experience.

This version positions the course as a premium professional training program, improves readability, and emphasizes practical industry value, which can help increase enrollments and perceived credibility.