Description

Chiral Separation Training by HPLC

Chiral Method Development Using Polysaccharide, Cyclodextrin & Crown Ether Stationary Phases

Master the science and strategy of chiral separations through this comprehensive industry-focused training program designed for analytical scientists, chromatographers, pharmaceutical researchers, and quality professionals. This advanced course provides a systematic approach to developing robust, cost-effective, and regulatory-compliant chiral methods using both HPLC and GC techniques.

Led by Dr. Pramod Kumar Pandey (PhD, Chemistry) with over 31 years of experience in global and Indian pharmaceutical industries, this program combines fundamental principles, practical method development workflows, real-world case studies, and expert troubleshooting strategies used in pharmaceutical R&D and quality control laboratories.

Whether you are new to chiral separations or looking to enhance your method development expertise, this training will equip you with the knowledge and confidence to tackle simple to highly complex chiral molecules.

What Makes This Training Unique?

• Complete coverage from chiral separation fundamentals to advanced method optimisation
• Structured decision-making approach for rapid method development
• Practical strategies for selecting columns, mobile phases, and modifiers
• Coverage of both Chiral HPLC and Chiral GC methodologies
• Real pharmaceutical case studies and troubleshooting examples
• Cost-reduction strategies for routine analytical operations
• Guidance on preparing professional chiral method development reports
• Includes all content from the Chiral Method Development Foundation Course

Training Modules

Module 1: Chiral Separation Fundamentals & Technique Selection

Build a strong foundation in chiral separation science and learn how to select the most appropriate analytical technique.

Topics covered:

• Fundamentals of chirality, enantiomers, diastereomers, and stereoisomers
• Principles and mechanisms of chiral recognition
• Three-point interaction model
• Understanding enantioselectivity
• Key chromatographic parameters:
  • Selectivity (α)
  • Resolution (Rs)
  • Retention Factor (k’)
  • Column Efficiency (N)
• Selecting between Chiral HPLC and Chiral GC
• Technique selection based on molecular structure and physicochemical properties
• Volatility, thermal stability, polarity, and molecular weight considerations
• Regulatory and analytical requirements
• Practical decision trees and real-world examples

Module 2: Chiral HPLC Method Development – Mobile Phase & Modifier Selection

Learn how to systematically develop and optimise chiral HPLC methods by selecting appropriate mobile phases, solvents, and modifiers.

Topics covered:

• Normal-phase chiral HPLC
• Reversed-phase chiral HPLC
• Polar-organic mode
• Heptane/alcohol mobile-phase systems
• Aqueous buffers and polar solvents
• Selection of alcohol modifiers
• Acidic and basic additives
• Polar modifiers and their applications
• Effect of mobile-phase composition on:
  • Selectivity
  • Retention
  • Resolution
  • Peak shape
• Modifier-related troubleshooting
• Improving method robustness and reproducibility
• Practical mobile-phase screening strategies

Module 3: Chiral Stationary Phase (CSP) Selection & Chiral GC

Develop a systematic approach for selecting the right chiral stationary phase based on analyte structure and separation requirements.

Topics covered:

• Polysaccharide-based CSPs
• Cyclodextrin-based CSPs
• Crown Ether CSPs
• Pirkle-type CSPs
• Protein-based CSPs
• Macrocyclic antibiotic CSPs
• Structure-based CSP selection
• Designing efficient chiral column screening workflows
• Chiral GC stationary phases
• Cyclodextrin-based GC chiral columns
• Derivatised cyclodextrin phases
• Specialised GC chiral stationary phases
• Selecting GC columns based on analyte volatility and functionality
• Temperature limitations and thermal stability
• Efficient Chiral GC screening strategies

Module 4: Chiral Method Development for Simple to Complex Molecules

Learn how to approach chiral separations systematically based on the number and arrangement of stereogenic centres.

Topics covered:

Molecules with One Chiral Centre

• Predicting chiral interactions
• Selecting appropriate CSPs and mobile phases
• Rapid first-pass screening strategies

Molecules with Two Chiral Centres

• Understanding enantiomeric and diastereomeric separations
• Managing multiple stereoisomers
• Resolving co-elution challenges

Molecules with Multiple Chiral Centres

• Advanced strategies for molecules containing 1, 2, and 3 chiral centres
• Complex pharmaceutical compounds
• Multi-column screening approaches
• Combining complementary separation techniques
• Optimising challenging chiral separations

Module 5: Chiral Method Optimisation, Troubleshooting & Cost Reduction

Transform a successful screening experiment into a robust, practical, and economical routine analytical method.

Topics covered:

• Structured method development workflow:
  Screen → Refine → Optimise → Validate
• Improving resolution and selectivity
• Optimising retention time and peak shape
• Troubleshooting:
  • Poor resolution
  • Co-elution
  • Low selectivity
  • Long retention times
  • Poor peak shape
  • Reproducibility issues
• Reducing solvent consumption
• Reducing analysis time
• Extending chiral column life
• Cost-effective column selection
• Efficient column and mobile-phase screening
• Developing economical routine methods
• Robustness studies
• Intermediate precision evaluation
• System suitability criteria
• Method lifecycle considerations

Module 6: Chiral Method Validation, Documentation & Real-World Case Studies

Learn how to finalise, document, and apply chiral methods in pharmaceutical analytical development and quality control environments.

Topics covered:

• Finalisation of routine chiral methods
• System suitability requirements
• Method robustness and reproducibility
• Chiral impurity analysis
• Enantiomeric purity determination
• Preparing professional chiral method development reports
• Column screening summaries
• Mobile-phase selection rationale
• Optimisation study documentation
• System suitability justification
• Final method reporting
• Regulatory expectations for pharmaceutical submissions
• Real pharmaceutical case studies
• Troubleshooting real-world chiral separation problems
• Frequently asked questions and practical problem-solving
• Strategies for applying course concepts to laboratory challenges

Training Details

Duration: 6 Live Online Sessions
Total Training Time: 9 Hours (540 Minutes)

Mode: Online Live Training via Google Meet

Certificate: Certificate of Completion Provided

Money-Back Guarantee: 30-Day Money-Back Guarantee

Language: English

Instructor: Dr. Pramod Kumar Pandey, PhD (Chemistry)
31+ Years of Experience in Global and Indian Pharmaceutical Industries

Learning Outcomes

Upon completion of this training, participants will be able to:

• Understand the fundamental principles of chiral recognition and separation
• Select appropriate chiral separation techniques for different molecules
• Choose suitable CSPs, mobile phases, and modifiers systematically
• Develop robust and efficient chiral HPLC and GC methods
• Troubleshoot complex separation challenges with confidence
• Reduce analytical costs while maintaining method quality
• Prepare professional chiral method development reports
• Implement validated routine chiral methods in pharmaceutical laboratories

Bonus

Exclusive discussion of real industrial chiral separation challenges, practical solutions, and expert insights gained from more than three decades of pharmaceutical experience.

This version positions the course as a premium professional training program, improves readability, and emphasizes practical industry value, which can help increase enrollments and perceived credibility.

Who Should Take This Chiral Separation Course?

This training is particularly useful for:

• Analytical Development Scientists
• HPLC and GC Analysts
• QC and QA Professionals
• Pharmaceutical R&D Scientists
• Method Development Scientists
• Analytical Chemists
• Chromatographers
• Pharmaceutical Researchers
• Regulatory Affairs Professionals
• CMC professionals
• Research Scholars and Academicians
• Professionals working with chiral APIs, intermediates, impurities, and stereoisomers

Whether you are learning chiral chromatography for the first time or already working on difficult chiral separations, the course provides a structured approach for developing practical and reliable methods.