Chiral Separation – Advanced Training | By HPLC & GC

Description

• 7+ detailed case studies covering:
  • One-centre and multi-centre chiral molecules
  • HPLC vs GC technique decisions
  • Troubleshooting poor selectivity, co-elution, and long runtime
  • Cost reduction examples
• Interactive FAQs with practical troubleshooting guidance.
• Bonus: Real industry challenges and expert solutions.

Chiral Separation – Advanced Training | By HPLC & GC

PharmaGuru Chiral Separation Training is an advanced, comprehensive program designed for industry professionals and researchers, focusing on chiral method development using HPLC and GC. The course covers everything from fundamentals of chirality and separation theory to column selection, mobile phase and modifier optimisation, method development strategies, and troubleshooting for simple to complex chiral molecules, delivered through structured training modules:

Module 1: Selecting the Right Chiral Separation Technique

• How to choose between Chiral HPLC and Chiral GC based on analyte properties, volatility, polarity, thermal stability, MW, and regulatory needs.
• Decision tree for technique selection with real-world examples.

Module 2: Principles of Chiral Method Development

• Fundamental concepts of enantioselectivity, chiral recognition, and asymmetric interactions.
• Key parameters: selectivity (α), resolution (Rs), efficiency (N), and retention (k’).
• Understanding three-point interaction models in chiral separations.

Module 3: Selecting Mobile Phase for Chiral HPLC

• How to choose normal-phase vs reversed-phase vs polar-organic modes.
• Rules for selecting heptane/alcohol ratios, aqueous buffers, and polar solvents.
• Mobile phase impact on selectivity, resolution, retention, and stability.

Module 4: Selecting Modifiers for Chiral HPLC

• Choosing the right alcohol, acid/base additives, and polar modifiers.
• How modifiers influence interaction mechanisms, peak shapes, and enantioselectivity.
• Troubleshooting issues caused by modifiers.

Module 5: Selecting the Right Chiral Column (CSP) for HPLC

• Overview of polysaccharide, Pirkle-type, cyclodextrin, protein, and macrocyclic antibiotic CSPs.
• How to select CSPs using structure-based screening and interaction mapping.
• How to design a column screening strategy to maximise success.

Module 6: Selecting CSPs for Chiral GC

• Choosing cyclodextrin-based, derivatised, and specialised GC chiral stationary phases.
• Selection based on volatility, functional groups, stability, and temperature limits.
• GC-specific chiral screening strategy.

Module 7: Structured Separation Strategies for Different Types of Chiral Molecules

a) Molecules with One Chiral Centre

• Predicting interactions and choosing the correct CSP and mobile phase.
• Simplified decision map for first-pass screening.

b) Molecules with Two Chiral Centres

• Understanding diastereomeric vs enantiomeric separation challenges.
• Strategy to handle multiple peaks and co-elution.

c) Molecules with Multiple Chiral Centres

• Handling complex stereochemical patterns.
• Advanced column pairing and multi-technique approaches.

Module 8: Reducing Analysis Cost in Chiral HPLC & GC

• Techniques to cut solvent usage, runtime, and column wear.
• How to select cost-efficient columns and mobile phases without sacrificing quality.
• Using common method strategies.

Module 9: Optimisation & Finalisation of Routine Chiral Methods

• Step-by-step optimisation workflow: screen → refine → optimise → validate.
• Robustness, intermediate precision, and system suitability criteria.
• Converting development methods into validated routine methods.

Module 10: Preparing a Chiral Method Development Report

• How to document:
  • Column screening results
  • Mobile phase rationale
  • Optimization experiments
  • System suitability justification
  • Final method summary
• Regulatory expectations for pharma-quality chiral methods.

Module 11: Case Studies, FAQs & Real-World Problem Solving

• 7+ detailed case studies covering:
  • One-centre and multi-centre chiral molecules
  • HPLC vs GC technique decisions
  • Troubleshooting poor selectivity, co-elution, and long runtime
  • Cost reduction examples
• Interactive FAQs with practical troubleshooting guidance.
• Bonus: Real industry challenges and expert solutions.