Validation of Pharmacopoeial Methods: How to Perform It Properly
Validation of pharmacopoeial methods should be performed using minimal analytical parameters, with a primary focus on specificity, detection limit, and quantification limit. Pharmacopoeial methods are presumed to be validated and therefore only require verification or partial validation. What is a Pharmacopoeial Method? Pharmacopoeial methods are analytical procedures published in recognised pharmacopoeias like: These methods are […]
Analytical Method Validation Protocol And Report: Key Differences & 3+ FAQs
Learn key differences between Validation protocol and report with frequently asked questions (FAQs) to help you better understand and implement them.
How is Signal-to-Noise Ratio Determined In HPLC, And What is its Role In DL and QL: Simple Tips
The signal-to-noise (S/N) ratio in HPLC is a key measure of analytical sensitivity. It is typically calculated using the formula: S/N = 2H/h. where H is the peak height and h is the noise height. In this blog post, I will discuss the concept of signal-to-noise ratio, how it’s determined, its role in establishing Detection […]
How to Read A Chromatogram In HPLC and GC: Peak, RT (Retention Time) and RRT
How to Read a Chromatogram is one of the fundamental skills to proceed with the HPLC and GC analysis. A chromatogram is interpreted by analysing the axes, identifying the peaks, and evaluating their characteristics such as Retention Time, Peak Shape, Peak Height and Area. In this article, I will explain how to read and interpret chromatograms, […]
What Is Chiral GC (Gas Chromatography): Learn With FAQs
Chiral Gas Chromatography (Chiral GC) is a powerful separation technique used to resolve enantiomers—molecules that are non-superimposable mirror images of each other—based on their differential interactions with a chiral stationary phase. Because different enantiomers of a compound can exhibit distinct biological activities, separating and controlling unwanted isomers is critical in the development of Active Pharmaceutical […]
What Is Supercritical Fluid Chromatography (SFC) And How It is Different From HPLC And GC
Discover Supercritical Fluid Chromatography (SFC) – a fast, eco-friendly technique for separating complex mixtures. Learn its principles, pharmaceutical applications, advantages, limitations, and how it compares to HPLC and GC.
Ion Exchange Chromatography (IEC): Quantification of Cations and Anions in APIs With 3+ FAQs
Learn how Ion Exchange Chromatography is used in pharmaceutical analysis for precise quantification of cations and anions in APIs. Includes principles, procedures, case studies, and regulatory insights.
What is DSC (Differential Scanning Calorimetry) And How Is It Useful In Drug Development
Explore how DSC aids drug development by analyzing thermal behavior, stability, and polymorphism
Quantification of Polymorphic Impurities in APIs Using XRPD: Case Study and 5+ FAQs
earn how to quantify polymorphic impurities in APIs using X-Ray Powder Diffraction (XRPD) with a step-by-step analytical approach, calibration formula, and a real-world Carbamazepine case study
Cross-Validation of Analytical Method: What It Is, How & When It’s Performed, and Why It Matters
Learn what cross-validation of an analytical method is, why it’s important, when to perform it, and how it’s done. Ensure method reliability, regulatory compliance, and data integrity across labs.